Abstract
Acquisition of tandem mass spectra from peptides or other analytes deposited on non-conductive membranes is inhibited on instruments combining matrix-assisted laser desorption/ionization with tandem time-of-flight analyzers (MALDI-TOF/TOF) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI-TOF/TOF systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Adsorption
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Coated Materials, Biocompatible / analysis
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Coated Materials, Biocompatible / chemistry*
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Electric Conductivity
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Gold / chemistry*
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Membranes, Artificial*
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Peptide Mapping / methods
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Protein Binding
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Proteins / analysis*
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Proteins / chemistry*
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Spectrometry, Mass, Electrospray Ionization / methods*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Substances
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Coated Materials, Biocompatible
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Membranes, Artificial
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Proteins
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Gold