Abstract
Effector cells armed with Abs can eliminate virus-infected target cells by Ab-dependent cellular cytotoxicity (ADCC), an immune mechanism that has been largely overlooked in HIV vaccine development. Here, we show that a prime/boost AIDS vaccine approach elicits potent ADCC activity correlating with protection against SIV in rhesus macaques (Macacca mulatta). Priming with replicating adenovirus type 5 host range mutant-SIV recombinants, followed by boosting with SIV gp120, elicited Abs with ADCC activity against SIV(mac251)-infected cells. In vitro ADCC activity correlated with in vivo reduced acute viremia after a mucosal challenge with pathogenic SIV. Our findings expose ADCC activity as an immune correlate that may be relevant in the rational design of an efficacious vaccine against HIV.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acute Disease
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Animals
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Antibodies, Viral / biosynthesis
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Antibodies, Viral / metabolism
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Antibodies, Viral / physiology*
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Antibody-Dependent Cell Cytotoxicity / immunology*
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Binding Sites, Antibody
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Cells, Cultured
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Gene Products, env / immunology
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Gene Products, env / metabolism
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Immunization, Secondary
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Immunoglobulin G / physiology
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Kinetics
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Leukocytes, Mononuclear / immunology
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Leukocytes, Mononuclear / virology
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Macaca mulatta
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Retrospective Studies
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SAIDS Vaccines / administration & dosage
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SAIDS Vaccines / immunology*
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SAIDS Vaccines / therapeutic use
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Simian Immunodeficiency Virus / immunology*
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / immunology
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Vaccines, Synthetic / therapeutic use
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Viral Load
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Viremia / immunology
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Viremia / prevention & control*
Substances
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Antibodies, Viral
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Gene Products, env
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Immunoglobulin G
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SAIDS Vaccines
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Vaccines, Synthetic