Clone-array pooled shotgun mapping and sequencing: design and analysis of experiments

Genome Inform. 2003:14:186-95.

Abstract

This paper studies sequencing and mapping methods that rely solely on pooling and shotgun sequencing of clones. First, we scrutinize and improve the recently proposed Clone-Array Pooled Shotgun Sequencing (CAPSS) method, which delivers a BAC-linked assembly of a whole genome sequence. Secondly, we introduce a novel physical mapping method, called Clone-Array Pooled Shotgun Mapping (CAPS-MAP), which computes the physical ordering of BACs in a random library. Both CAPSS and CAPS-MAP construct subclone libraries from pooled genomic BAC clones.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chromosomes, Artificial, Bacterial
  • Computational Biology / methods*
  • Computer Simulation
  • Drosophila melanogaster / genetics
  • Genome
  • Reproducibility of Results
  • Research Design
  • Sequence Alignment