Abstract
Objective:
To observe the different effects of Puerarin and Daidzein on the expression of proliferating vascular smooth muscle cells, and to discuss the mechanism.
Method:
MT was used to detect the state of VSMC (vascular smooth muscle cell) activity. The expression levels of Survivin, Bcl-xl, Bax and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) messenger RNA (mRNA) were analyzed quantitatively by reverse transcriptase polymerase chain reaction (Rt-PCR).
Result:
Compared with Puerarin groups, VSMC activity in daidzein groups was lower, and the ratio of Bax/Gapdh/Bcl-xl/Gapdh was higher.
Conclusion:
The inhibition effect of daidzein on VSMC proliferation is stronger than that of puerarin.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Cells, Cultured
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Gene Expression Regulation / drug effects
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Glyceraldehyde-3-Phosphate Dehydrogenases / biosynthesis
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Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
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Humans
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Inhibitor of Apoptosis Proteins
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Isoflavones / isolation & purification
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Isoflavones / pharmacology*
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Microtubule-Associated Proteins / biosynthesis
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Microtubule-Associated Proteins / genetics
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Muscle, Smooth, Vascular / cytology
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Myocytes, Smooth Muscle / drug effects
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Myocytes, Smooth Muscle / metabolism*
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Neoplasm Proteins
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Plants, Medicinal / chemistry
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Proto-Oncogene Proteins c-bcl-2 / biosynthesis*
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Proto-Oncogene Proteins c-bcl-2 / genetics
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Pueraria / chemistry
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Survivin
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Vasodilator Agents / pharmacology
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bcl-2-Associated X Protein
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bcl-X Protein
Substances
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BAX protein, human
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BCL2L1 protein, human
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BIRC5 protein, human
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Inhibitor of Apoptosis Proteins
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Isoflavones
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Microtubule-Associated Proteins
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Neoplasm Proteins
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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Survivin
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Vasodilator Agents
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bcl-2-Associated X Protein
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bcl-X Protein
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daidzein
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Glyceraldehyde-3-Phosphate Dehydrogenases
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puerarin