Chloramphenicol and expression of multidrug efflux pump in Enterobacter aerogenes

Biochem Biophys Res Commun. 2005 Mar 25;328(4):1113-8. doi: 10.1016/j.bbrc.2005.01.069.

Abstract

Chloramphenicol has been reported to act as an inducer of the multidrug resistance in Escherichia coli. A resistant variant able to grow on plates containing 64 microg/ml chloramphenicol was obtained from the Enterobacter aerogenes ATCC 13048-type strain. Chloramphenicol resistance was due to an active efflux of this antibiotic and it was associated with resistance to fluoroquinolones and tetracycline, but not to aminoglycoside or beta-lactam antibiotics. MDR in the chloramphenicol-resistant variant is linked to the overexpression of the major AcrAB-TolC efflux system. This overexpression seems unrelated to the global Mar and the local AcrR regulatory pathways.

Publication types

  • Comparative Study

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B / drug effects
  • ATP Binding Cassette Transporter, Subfamily B / metabolism
  • Anti-Bacterial Agents / pharmacokinetics
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / antagonists & inhibitors*
  • Bacterial Proteins / metabolism*
  • Carrier Proteins / antagonists & inhibitors*
  • Carrier Proteins / metabolism*
  • Chloramphenicol / pharmacokinetics*
  • Chloramphenicol / pharmacology*
  • Dose-Response Relationship, Drug
  • Drug Resistance, Multiple, Bacterial / drug effects
  • Drug Resistance, Multiple, Bacterial / physiology*
  • Enterobacter aerogenes / classification
  • Enterobacter aerogenes / drug effects*
  • Enterobacter aerogenes / metabolism*
  • Gene Expression Regulation, Bacterial / drug effects
  • Metabolic Clearance Rate
  • Species Specificity

Substances

  • ATP Binding Cassette Transporter, Subfamily B
  • AcrAB-TolC protein, Salmonella enterica
  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carrier Proteins
  • Chloramphenicol