Antibody neutralization of vascular endothelial growth factor (VEGF) fails to attenuate vascular permeability and brain edema in experimental pneumococcal meningitis

J Neuroimmunol. 2005 Mar;160(1-2):170-7. doi: 10.1016/j.jneuroim.2004.11.013.

Abstract

To determine the contribution of vascular endothelial growth factor (VEGF) to cerebral edema formation in bacterial meningitis, we used a VEGF neutralizing antibody to block VEGF in rabbits, following induction of meningitis by intracisternal inoculation with 10(9) heat-killed pneumococci. At 8 h, cerebrospinal fluid (CSF) VEGF was significantly elevated in infected untreated animals, and correlated with CSF white blood cell (WBC) count (r=0.56, P=0.004), and brain water content (r=0.42, P=0.04). Blocking of VEGF did not attenuate brain edema, blood-brain barrier disruption, or CSF pleocytosis. The functional role of VEGF in the pathophysiology of BM remains elusive.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Blocking / administration & dosage*
  • Antibodies, Blocking / pharmacology
  • Antibodies, Monoclonal / administration & dosage
  • Antibodies, Monoclonal / pharmacology
  • Antibodies, Monoclonal, Humanized
  • Bevacizumab
  • Brain Edema / blood
  • Brain Edema / cerebrospinal fluid
  • Brain Edema / immunology*
  • Brain Edema / physiopathology*
  • Capillary Permeability / immunology*
  • Cell Movement / immunology
  • Cisterna Magna
  • Female
  • Humans
  • Injections, Intravenous
  • Leukocytes / immunology
  • Leukocytes / pathology
  • Meningitis, Pneumococcal / blood
  • Meningitis, Pneumococcal / cerebrospinal fluid
  • Meningitis, Pneumococcal / immunology*
  • Meningitis, Pneumococcal / physiopathology*
  • Mice
  • Rabbits
  • Vascular Endothelial Growth Factor A / antagonists & inhibitors*
  • Vascular Endothelial Growth Factor A / blood
  • Vascular Endothelial Growth Factor A / cerebrospinal fluid
  • Vascular Endothelial Growth Factor A / immunology*
  • Water-Electrolyte Balance

Substances

  • Antibodies, Blocking
  • Antibodies, Monoclonal
  • Antibodies, Monoclonal, Humanized
  • Vascular Endothelial Growth Factor A
  • Bevacizumab