Protein heterodimerization through ligand-bridged multivalent pre-organization: enhancing ligand binding toward both protein targets

J Am Chem Soc. 2005 Feb 23;127(7):2044-5. doi: 10.1021/ja043817r.

Abstract

Structure-based design of a bifunctional ligand for two protein pentamers, cholera toxin B pentamer (CTB) and human serum amyloid P component (SAP), leads to multivalent dimerization of CTB and SAP in solution. This multivalent heterodimerization of proteins significantly enhances the affinity of the bifunctional ligand toward both target proteins.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cholera Toxin / chemistry*
  • Dimerization
  • Kinetics
  • Ligands
  • Proline / analogs & derivatives*
  • Proline / chemistry
  • Protein Binding
  • Serum Amyloid P-Component / chemistry*
  • Structure-Activity Relationship

Substances

  • Ligands
  • Serum Amyloid P-Component
  • Cholera Toxin
  • Proline
  • N-acetylproline