Abstract
The strategy of combined site directed mutagenesis and chemical modification with polar prosthetic groups was used to broaden substrate specificity of proteases resulting in the first successful formation of glycopeptides through the use of glucoamino acid acyl donors in yields of up to 90%.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Glycolipids / chemistry
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Glycolipids / metabolism
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Glycopeptides / chemistry
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Glycopeptides / metabolism*
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Molecular Structure
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Mutagenesis, Site-Directed
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Peptide Hydrolases / chemistry
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Peptide Hydrolases / genetics
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Peptide Hydrolases / metabolism*
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Substrate Specificity
Substances
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Glycolipids
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Glycopeptides
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Peptide Hydrolases