Copper-mediated intra-ligand oxygen transfer in gas-phase complexes with 3-nitrotyrosine

J Mass Spectrom. 2005 May;40(5):608-14. doi: 10.1002/jms.829.

Abstract

Gas-phase ternary complexes with Cu(II) and 2,2'-bipyridine (bpy) of tyrosine, 3-aminotyrosine, 3-nitrotyrosine and 3-nitrotyrosine methyl ether are formed readily upon electrospraying aqueous methanol solutions containing the components. In contrast to Cu(bpy) complexes of tyrosine, 3-aminotyrosine and other aromatic amino acids, the complexes of 3-nitrotyrosine and its methyl ether undergo unusual collisionally activated dissociations (CADs) that involve Cu-mediated transfer of an oxygen atom from the nitro group. With 3-nitrotyrosine this results in an expulsion of carbonic acid, H(2)CO(3), whereas with 3-nitrotyrosine methyl ether an OH migration forms Cu(OH)bpy(+) as the predominant product. To the best of our knowledge, this is the first case of an intra-ligand redox reaction in a gas-phase organometallic complex. The reaction mechanism of this unusual dissociation was elucidated by a combination of isotope labeling, accurate mass measurements, energy-resolved CAD mass spectra and density functional theory calculations of ion structures and relative energies.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Carbonic Acid / chemistry
  • Copper / chemistry*
  • Deuterium Exchange Measurement
  • Gases / chemistry*
  • Ions / chemistry
  • Ligands
  • Macromolecular Substances
  • Molecular Structure
  • Oxygen / chemistry*
  • Spectrometry, Mass, Electrospray Ionization
  • Tyrosine / analogs & derivatives*
  • Tyrosine / chemistry*

Substances

  • Gases
  • Ions
  • Ligands
  • Macromolecular Substances
  • 3-nitrotyrosine
  • Tyrosine
  • Carbonic Acid
  • Copper
  • Oxygen