Mupirocin W, a novel pseudomonic acid produced by targeted mutation of the mupirocin biosynthetic gene cluster

Sian M Cooper; Russell J Cox; John Crosby; Matthew P Crump; Joanne Hothersall; Wanpen Laosripaiboon; Thomas J Simpson; Christopher M Thomas

doi:10.1039/b414781b

Mupirocin W, a novel pseudomonic acid produced by targeted mutation of the mupirocin biosynthetic gene cluster

Chem Commun (Camb). 2005 Mar 7:(9):1179-81. doi: 10.1039/b414781b. Epub 2005 Jan 17.

Authors

Sian M Cooper¹, Russell J Cox, John Crosby, Matthew P Crump, Joanne Hothersall, Wanpen Laosripaiboon, Thomas J Simpson, Christopher M Thomas

Affiliation

¹ School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK.

PMID: 15726184
DOI: 10.1039/b414781b

Abstract

Mutation of the mupW gene in the mupirocin biosynthetic gene cluster in Pseudomonas fluorescens results in efficient production of a novel pseudomonic acid metabolite, mupirocin W, which lacks the characteristic tetrahydropyran ring, and reveals the role of the mupW gene in pseudomonic acid biosynthesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / biosynthesis*
Molecular Structure
Multigene Family / genetics*
Mupirocin / biosynthesis*
Mutagenesis, Site-Directed*
Pseudomonas fluorescens / genetics
Pseudomonas fluorescens / metabolism*

Substances

Anti-Bacterial Agents
Mupirocin