Mycobacterium tuberculosis with disruption in genes encoding the phosphate binding proteins PstS1 and PstS2 is deficient in phosphate uptake and demonstrates reduced in vivo virulence

Priska Peirs; Philippe Lefèvre; Samira Boarbi; Xiao-Ming Wang; Olivier Denis; Martine Braibant; Kevin Pethe; Camille Locht; Kris Huygen; Jean Content

doi:10.1128/IAI.73.3.1898-1902.2005

Mycobacterium tuberculosis with disruption in genes encoding the phosphate binding proteins PstS1 and PstS2 is deficient in phosphate uptake and demonstrates reduced in vivo virulence

Infect Immun. 2005 Mar;73(3):1898-902. doi: 10.1128/IAI.73.3.1898-1902.2005.

Authors

Priska Peirs¹, Philippe Lefèvre, Samira Boarbi, Xiao-Ming Wang, Olivier Denis, Martine Braibant, Kevin Pethe, Camille Locht, Kris Huygen, Jean Content

Affiliation

¹ Pasteur Institute of Brussels, Engelandstraat 642, B-1180-Brussels, Belgium.

Abstract

By measuring phosphate uptake by Mycobacterium tuberculosis strains with the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding proteins. In a mouse infection model, both mutants were attenuated in virulence, suggesting that M. tuberculosis encounters limiting phosphate concentrations during its intracellular life span.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Culture Media
Gene Deletion*
Humans
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / growth & development*
Mycobacterium tuberculosis / metabolism
Mycobacterium tuberculosis / pathogenicity*
Phosphate-Binding Proteins / genetics*
Phosphate-Binding Proteins / metabolism
Phosphates / metabolism*
Tuberculosis / microbiology*
Tuberculosis / physiopathology
Virulence

Substances

Bacterial Proteins
Culture Media
Phosphate-Binding Proteins
Phosphates