Increased plasma levels of urokinase plasminogen activator and matrix metalloproteinase-9 in nonsmall cell lung cancer patients

Clin Chim Acta. 2005 Apr;354(1-2):91-9. doi: 10.1016/j.cccn.2004.11.011. Epub 2004 Dec 23.

Abstract

Background: The involvements of matrix metalloproteinase-9 (MMP-9) and urokinase plasminogen activator (uPA) in the pathogenesis of various types of lung cancers have been established. The aim of the present study was to determine the concentrations of MMP-9 and uPA in the plasma of nonsmall cell lung cancer (NSCLC) patients.

Methods: Gelatin zymography and ELISA were used to measure MMP-9 and uPA concentrations, respectively, in 90 NSCLC patients and 50 control subjects.

Results: Compared with that of control subjects, MMP-9 and uPA levels were significantly higher in the plasma of NSCLC patients (P<0.001 and P<0.01, respectively). Further statistical analysis for clinic pathological parameters revealed that MMP-9 and uPA levels were significantly increased in patients with metastasis (P<0.01 and P<0.05, respectively). Furthermore, the MMP-9 level was significantly different between smokers and nonsmokers (P<0.05) while uPA levels varied between histological types (P<0.05).

Conclusions: Plasma MMP-9 and uPA might play an important role in the metastasis of NSCLC by involvement in the processes of invasion and metastasis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Carcinoma, Non-Small-Cell Lung / enzymology*
  • Cell Line, Tumor
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Gelatin
  • Humans
  • Lung Neoplasms / enzymology*
  • Male
  • Matrix Metalloproteinase 9 / analysis
  • Matrix Metalloproteinase 9 / blood*
  • Matrix Metalloproteinase 9 / metabolism
  • Middle Aged
  • Urokinase-Type Plasminogen Activator / analysis
  • Urokinase-Type Plasminogen Activator / blood*
  • Urokinase-Type Plasminogen Activator / metabolism

Substances

  • Gelatin
  • Urokinase-Type Plasminogen Activator
  • Matrix Metalloproteinase 9