Laparoscopic seromuscular colonic biopsies: a surgeon's experience

J Pediatr Surg. 2005 Feb;40(2):381-4. doi: 10.1016/j.jpedsurg.2004.10.031.

Abstract

Background: Colonic dysmotility is a recognised cause of chronic constipation in children. Colonic dysmotility is better analysed by examination of the colonic muscle than rectal biopsy, which does not examine the defective area and has a low yield. We explored the role of laparoscopic colonic muscle biopsies to investigate children with intractable constipation. The authors describe the technique, its application, and results from a large series.

Methods: A retrospective review was conducted of all patients undergoing laparoscopic seromuscular colonic biopsies (hepatic flexure, mid-transverse colon, splenic flexure, and sigmoid colon) by a single surgeon for the investigation of chronic constipation over a 10-year period. Patient records were reviewed to determine the perforation frequency and management, postoperative recovery time and the frequency of an immunohistochemical abnormality.

Results: One hundred ninety-seven patients (118 boys) were investigated by laparoscopic biopsy during the period. The mean age was 8.0 +/- 4.0 years (range, 1.4-22.4). The patients took 28.7 +/- 13.6 hours (range, 8-120) to recover, with 37 (19%) having nausea and/or vomiting requiring antiemetics. Most patients (160/197, 81%) were discharged the following day. Six patients (3%) had a mucosal perforation recognised at operation (treated by an Endoloop) with no change in postoperative outcome. Two patients (1%) had an unrecognised mucosal perforation requiring laparoscopic reoperation and Endoloop closure (laparotomy/colostomy not required), with no further sequelae. Eight-six patients (44%) had a specific immunohistochemical neuropeptide anomaly (reduced substance P [84], reduced vasoactive intestinal peptide [2]).

Conclusions: Laparoscopic biopsy is a valuable tool to investigate chronic constipation in children, allowing a pathological diagnosis to be made in many cases. The complications of the procedure are acceptably low with this technique.

MeSH terms

  • Adolescent
  • Adult
  • Biopsy / methods*
  • Child
  • Child, Preschool
  • Chronic Disease
  • Colon / pathology*
  • Constipation / pathology*
  • Enteric Nervous System / pathology
  • Female
  • Humans
  • Infant
  • Intestinal Mucosa / injuries
  • Intestinal Mucosa / innervation
  • Laparoscopy*
  • Male
  • Muscle, Smooth / innervation
  • Muscle, Smooth / pathology
  • Retrospective Studies