Abstract
To investigate the mechanism of phytochrome action in vivo, NtPHYB, AtPHYB and phyD:green fluorescent protein (GFP) were overexpressed in Nicotiana plumbaginifolia and Arabidopsis thaliana. The expression of 35S:NtPHYB:GFP and 35S:AtPHYB:GFP complemented the tobacco hgl2 and Arabidopsis phyB-9 mutations, whereas the 35S:AtPHYD:GFP only rescued the hgl2 mutant. All three fusion proteins are transported into the nucleus in all genetic backgrounds. These data indicate that AtPHYD:GFP is biologically active and functions as the main red light receptor in transgenic tobacco, and establish an experimental system for the functional analysis of this elusive photoreceptor in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus / physiology
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Apoproteins / genetics
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Apoproteins / metabolism*
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Gene Expression Regulation, Plant / genetics*
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Green Fluorescent Proteins
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Light Signal Transduction / genetics*
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Mutation / genetics
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Nicotiana / genetics
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Nicotiana / metabolism*
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Photoreceptor Cells / metabolism*
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Photosynthetic Reaction Center Complex Proteins / genetics
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Photosynthetic Reaction Center Complex Proteins / metabolism*
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Phytochrome / genetics
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Phytochrome / metabolism*
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Phytochrome B
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Recombinant Fusion Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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Apoproteins
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Arabidopsis Proteins
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PHYB protein, Arabidopsis
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Photosynthetic Reaction Center Complex Proteins
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Plant Proteins
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Recombinant Fusion Proteins
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Transcription Factors
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Phytochrome
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Phytochrome B
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Green Fluorescent Proteins
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PHYD protein, Arabidopsis