Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus

Maofeng Qiu; Jin Wang; Hongxia Wang; Zeliang Chen; Erhei Dai; Zhaobiao Guo; Xiaoyi Wang; Xin Pang; Baoxing Fan; Jie Wen; Jian Wang; Ruifu Yang

doi:10.1128/CDLI.12.3.474-476.2005

Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus

Clin Diagn Lab Immunol. 2005 Mar;12(3):474-6. doi: 10.1128/CDLI.12.3.474-476.2005.

Authors

Maofeng Qiu¹, Jin Wang, Hongxia Wang, Zeliang Chen, Erhei Dai, Zhaobiao Guo, Xiaoyi Wang, Xin Pang, Baoxing Fan, Jie Wen, Jian Wang, Ruifu Yang

Affiliation

¹ Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, People's Republic of China.

Abstract

Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral / analysis
Binding Sites
Coronavirus / chemistry
Coronavirus / isolation & purification
Enzyme-Linked Immunosorbent Assay / methods*
Enzyme-Linked Immunosorbent Assay / standards
Humans
Nucleocapsid / analysis*
Respiratory Distress Syndrome / diagnosis*
Sensitivity and Specificity
Serologic Tests / methods

Substances

Antibodies, Viral