Expression of cell adhesion molecules and chemokine receptors: angioinvasiveness in nasal NK/T-cell lymphoma

Oncol Rep. 2005 Apr;13(4):613-20.

Abstract

Sinonasal natural killer (NK)/T-cell lymphoma (NKTCL) is closely associated with Epstein-Barr virus (EBV) infection and expresses latent membrane protein (LMP)-1 and EB nuclear antigen (EBNA)-1, i.e., latency II of EBV infection. Angioinvasion by neoplastic cells is a characteristic feature of NKTCL, but its mechanism is unknown. To elucidate the molecular mechanism of angio-invasiveness in NKTCL, expression of cell adhesion molecules and chemokine receptors at mRNA and protein levels was examined using real-time PCR and immunohistochemistry in 17 NKTCL together with 10 diffuse large B-cell lymphoma (DLBL) and 9 non-neoplastic nasal mucosa as controls. EBV DNA was detected in 14 of 16 NKTCL examined, and 7 of these 14 expressed LMP-1. mRNA expression levels of integrin subunits alpha4, alpha L, alpha M, and beta2 were significantly higher in NKTCL than non-neoplastic controls. Integrin subunits alpha2 and alpha M were expressed at a significantly higher level in NKTCL with angioinvasion than those without. Expression level of alpha M was significantly higher in 7 cases of NKTCL with LMP-1 expression than 7 without. Immunohistochemistry showed expression of these molecules in NKTCL cells. These findings suggest that EBV infection might be involved in the pathogenesis of angioinvasion of NKTCL through up-regulation of alpha M by LMP-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, Viral / chemistry
  • Cell Adhesion
  • Cell Line, Tumor
  • DNA Primers / chemistry
  • DNA, Complementary / metabolism
  • Epstein-Barr Virus Nuclear Antigens / biosynthesis
  • Female
  • Humans
  • Immunohistochemistry
  • Immunophenotyping
  • In Situ Hybridization
  • Killer Cells, Natural / pathology*
  • Lymphoma, T-Cell / metabolism*
  • Lymphoma, T-Cell / pathology*
  • Male
  • Middle Aged
  • Models, Biological
  • Nasal Mucosa / pathology
  • Neovascularization, Pathologic*
  • RNA / metabolism
  • RNA, Messenger / metabolism
  • Receptors, Chemokine / biosynthesis*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Up-Regulation
  • Viral Matrix Proteins / biosynthesis
  • Viral Proteins / chemistry
  • Virus Latency

Substances

  • Antigens, Viral
  • DNA Primers
  • DNA, Complementary
  • EBV-associated membrane antigen, Epstein-Barr virus
  • Epstein-Barr Virus Nuclear Antigens
  • RNA, Messenger
  • Receptors, Chemokine
  • Viral Matrix Proteins
  • Viral Proteins
  • RNA