Abstract
The mouse polymerase delta-interacting protein 1 gene, PDIP1, is mapped to chromosome 7F3 region, spans approximately 16.7kb, and is organized into six exons. The transcription start site (TSS) was determined to be G, corresponding to position of 162-bp upstream of the translation start codon. The promoter region was found to lack TATA box or CCAAT box, instead, a CpG island was detected surrounding TSS. The region from -162 to +114 is required for basal transcriptional regulation of mouse PDIP1 gene, contains two AP-2 and two Sp1 binding sites. The Sp1 site upstream of TSS activates, while the other Sp1 site and two AP-2 sites suppress the transcription activity of mouse PDIP1 gene.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Carrier Proteins / genetics*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology*
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Down-Regulation
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Exons / genetics
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Gene Components
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Genome
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Mice
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Molecular Sequence Data
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Promoter Regions, Genetic / genetics*
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Sp1 Transcription Factor / genetics
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Sp1 Transcription Factor / physiology*
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Transcription Factor AP-2
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Transcription Initiation Site
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Transcription, Genetic
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Ubiquitin-Protein Ligase Complexes
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Up-Regulation
Substances
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Carrier Proteins
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DNA-Binding Proteins
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Sp1 Transcription Factor
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Transcription Factor AP-2
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Transcription Factors
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PDIP1 protein, mouse
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Ubiquitin-Protein Ligase Complexes