Optimal bedside urinalysis for the detection of proteinuria in hypertensive pregnancy: a study of diagnostic accuracy

BJOG. 2005 Apr;112(4):412-7. doi: 10.1111/j.1471-0528.2004.00455.x.

Abstract

Objective: To compare semi-quantitative visual and automated methods of urine testing with fully quantitative point of care urinalysis for the detection of significant proteinuria (0.3 g/24 hours) in pregnancy complicated by hypertension.

Design: A prospective comparative study.

Setting: A large teaching maternity hospital.

Sample: One hundred and seventy-one pregnant women referred to the obstetric day-care unit for assessment of newly arisen hypertension.

Methods: Early morning urine specimens were tested with four dipstick techniques (Multistix 8SG visual and automated and microalbumin/creatinine ratio visual and automated; Bayer, Elkhart, USA) as well as a fully quantitative measure of the microalbumin creatinine ratio with the DCA 2000 (a point of care assay for albumin; Bayer). These results were compared to a 24-hour urine protein measurement and measures of diagnostic accuracy/prediction are reported.

Main outcome measures: Significant proteinuria (> or =0.3 g/24 hours) measured by laboratory assay.

Results: Automated dipstick urinalysis using the Clinitek 50 has significantly better predictive values for significant proteinuria (LR(+) 4.27, 95% CI 2.78 to 6.56; LR(-) 0.225, 95% CI 0.14 to 0.37) than conventional visual dipstick urinalysis (LR(+) 2.27, 95% CI 1.47 to 3.51; LR(-) 0.635, 95% CI 0.49 to 0.82). Dipstick microalbumin/creatinine ratio testing did not improve overall detection rates with automated or visual testing. Fully quantitative point of care measurement of albumin/creatinine ratio (ACR) was significantly better than any dipstick technique (LR(+) 14.6, 95% CI 6.74 to 31.8; LR(-) 0.069, 95% CI 0.030 to 0.16).

Conclusions: This study confirms that in pregnancy automated dipstick urinalysis is a more accurate screening test for the detection of proteinuria than visual testing. ACR testing can offer a significant improvement over conventional urinalysis if a fully quantitative method of detection is employed that uses pregnancy-specific thresholds. Dipstick assessment of ACR does not improve the detection rate of significant proteinuria.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adult
  • Day Care, Medical
  • Female
  • Humans
  • Hypertension, Pregnancy-Induced / urine*
  • Point-of-Care Systems / standards*
  • Pregnancy
  • Prenatal Diagnosis / standards*
  • Prospective Studies
  • Proteinuria / diagnosis*
  • ROC Curve
  • Reagent Strips / standards
  • Sensitivity and Specificity
  • Urinalysis / standards*

Substances

  • Reagent Strips