[Analysis of trichothecenes in barley tea and beer by liquid chromatography/tandem mass spectrometry]

Shokuhin Eiseigaku Zasshi. 2004 Dec;45(6):307-12. doi: 10.3358/shokueishi.45.307.
[Article in Japanese]

Abstract

A simple method for analysis of trichothecenes [Type A: diacetoxyscirpenol, neosolaniol, HT-2 toxin, and T-2 toxin, Type B: deoxynivalenol, nivalenol, fusarenon-X, 3-acetyldeoxynivalenol, 15-acetyldeoxynivarenol] in barley tea and beer using liquid chromatography tandem mass spectrometry (LC/MS/MS) was developed. Trichothecenes were extracted with ethyl acetate-methanol (19:1). The solvent was evaporated to dryness and the residue was dissolved in water-methanol (3:1) for injection into the LC/MS/MS. The LC separation was performed with an octadecylated silica column at a flow-rate of 0.2 mL/min, using a mobile phase consisting of water, methanol and acetonitrile. MS/MS was used in multiple reaction monitoring, employing electrospray ionization (ESI-MRM). The recoveries of trichothecenes from drinks at 1 microg/L (Type A) and 10 microg/L (Type B) were 52.5-115.2% (barley tea) and 68.1-127.5% (beer). Five barley tea and ten beer samples were analyzed by this method. Trichothecenes were not detected in them. This method may have applications in quality assurance.

Publication types

  • English Abstract

MeSH terms

  • Beer / analysis*
  • Chromatography, Liquid / methods*
  • Food Analysis / methods*
  • Food Contamination / analysis*
  • Hordeum / chemistry*
  • Hordeum / microbiology
  • Mass Spectrometry / methods*
  • Trichothecenes / analysis*

Substances

  • Trichothecenes