Protein diversity is generated within the motin family of proteins by alternative pre-mRNA splicing

Julie Moreau; Mathieu Lord; Maryse Boucher; Pascal Belleau; Maria J G Fernandes

doi:10.1016/j.gene.2005.02.001

Protein diversity is generated within the motin family of proteins by alternative pre-mRNA splicing

Gene. 2005 May 9;350(2):137-48. doi: 10.1016/j.gene.2005.02.001. Epub 2005 Mar 29.

Authors

Julie Moreau¹, Mathieu Lord, Maryse Boucher, Pascal Belleau, Maria J G Fernandes

Affiliation

¹ Centre de recherche en rhumatologie et immunologie, Centre de recherche du CHUL, Laval University, Bloc T1-492705, Boul. Laurier, Ste-Foy, Québec, Canada G1V 4G2.

PMID: 15804419
DOI: 10.1016/j.gene.2005.02.001

Abstract

The motin family of proteins is comprised of three polypeptides, angiomotin, angiomotin-like 1, and angiomotin-like 2. Angiomotin is an angiostatin-binding protein that promotes endothelial cell motility and is involved in angiogenesis. The function of human angiomotin-like-1 and angiomotin-like-2, however, remains unknown. In this report, we investigated the potential for molecular diversity within the motin family of proteins through the identification and characterization of alternatively spliced transcripts in endothelial cells, human tissues and a variety of cell lines. We report that the motins display variability at the mRNA level suggesting an intricate regulatory system at the transcriptional and potentially protein level. Some alternative transcripts are expressed in a tissue-specific manner and others give rise to novel protein isoforms. The alternative splicing that occurs within this protein family may have important implications in the regulation of the expression and function of the motins.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Alternative Splicing*
Angiomotins
Blotting, Northern
Carrier Proteins / genetics*
Carrier Proteins / metabolism
Cell Line
Cell Line, Tumor
Exons
Female
Gene Expression Profiling
Genes / genetics
HT29 Cells
HeLa Cells
Humans
Intercellular Signaling Peptides and Proteins / genetics*
Intercellular Signaling Peptides and Proteins / metabolism
Introns
K562 Cells
Membrane Proteins
Microfilament Proteins
Microscopy, Fluorescence
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA Precursors / genetics*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Deletion
Transfection
U937 Cells

Substances

AMOT protein, human
Angiomotins
Carrier Proteins
Intercellular Signaling Peptides and Proteins
Membrane Proteins
Microfilament Proteins
Protein Isoforms
RNA Precursors
RNA, Messenger
Recombinant Fusion Proteins