Identification of 40LoVe, a Xenopus hnRNP D family protein involved in localizing a TGF-beta-related mRNA during oogenesis

Kevin Czaplinski; Thomas Köcher; Malgorzata Schelder; Alexandra Segref; Matthias Wilm; Iain W Mattaj

doi:10.1016/j.devcel.2005.01.012

Identification of 40LoVe, a Xenopus hnRNP D family protein involved in localizing a TGF-beta-related mRNA during oogenesis

Dev Cell. 2005 Apr;8(4):505-15. doi: 10.1016/j.devcel.2005.01.012.

Authors

Kevin Czaplinski¹, Thomas Köcher, Malgorzata Schelder, Alexandra Segref, Matthias Wilm, Iain W Mattaj

Affiliation

¹ EMBL Gene Expression Programme, Meyerhofstrasse 1, Heidelberg 69117, Germany.

PMID: 15809033
DOI: 10.1016/j.devcel.2005.01.012

Abstract

Asymmetric distribution of cellular components underlies many biological processes, and the localization of mRNAs within domains of the cytoplasm is one important mechanism of establishing and maintaining cellular asymmetry. mRNA localization often involves assembly of large ribonucleoproteins (RNPs) in the cytoplasm. Using an RNA affinity chromatography approach, we investigated localization RNP formation on the vegetal localization element (VLE) of the mRNA encoding Vg1, a Xenopus TGF-beta family member. We identified 40LoVe, an hnRNP D family protein, as a specific VLE binding protein from Xenopus oocytes. Interaction of 40LoVe with the VLE strictly correlates with the ability of the RNA to localize, and antibodies against 40LoVe inhibit vegetal localization in vivo in oocytes. Our results associate an hnRNP D protein with mRNA localization and have implications for several functions mediated by this important protein family.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Chromatography, Affinity / methods
Glycoproteins / genetics
Glycoproteins / metabolism*
Heterogeneous-Nuclear Ribonucleoprotein D / classification
Heterogeneous-Nuclear Ribonucleoprotein D / genetics
Heterogeneous-Nuclear Ribonucleoprotein D / metabolism*
Heterogeneous-Nuclear Ribonucleoproteins / classification
Heterogeneous-Nuclear Ribonucleoproteins / genetics
Heterogeneous-Nuclear Ribonucleoproteins / metabolism*
Molecular Sequence Data
Oocytes / cytology
Oocytes / metabolism
Oogenesis / physiology*
Phylogeny
Protein Binding
RNA, Messenger / metabolism*
Sequence Alignment
Transforming Growth Factor beta / genetics
Transforming Growth Factor beta / metabolism*
Xenopus Proteins / classification
Xenopus Proteins / genetics
Xenopus Proteins / metabolism*
Xenopus laevis / genetics
Xenopus laevis / metabolism

Substances

GDF1 protein, Xenopus
Glycoproteins
HNRNPAB protein, Xenopus
Heterogeneous-Nuclear Ribonucleoprotein D
Heterogeneous-Nuclear Ribonucleoproteins
RNA, Messenger
Transforming Growth Factor beta
Xenopus Proteins