Nucleotide and derived amino acid sequence of the subtilisin from the antarctic psychrotroph Bacillus TA39

E Narinx; S Davail; G Feller; C Gerday

doi:10.1016/0167-4781(92)90108-c

Nucleotide and derived amino acid sequence of the subtilisin from the antarctic psychrotroph Bacillus TA39

Biochim Biophys Acta. 1992 May 7;1131(1):111-3. doi: 10.1016/0167-4781(92)90108-c.

Authors

E Narinx¹, S Davail, G Feller, C Gerday

Affiliation

¹ Laboratory of Biochemistry, University of Liège, Institute of Chemistry, Belgium.

PMID: 1581352
DOI: 10.1016/0167-4781(92)90108-c

Abstract

The nucleotide sequence of the subtilisin-encoding gene from the antarctic psychrotroph Bacillus TA39 was determined. The primary structure of the subtilisin precursor is composed of 420 amino acids giving rise to a mature enzyme of 309 amino acids. Asp-145, His-185 and Ser-361 are the proposed catalytic residues of the active site.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacillus / genetics*
Base Sequence
Cloning, Molecular
Genes, Bacterial*
Molecular Sequence Data
Protein Precursors / genetics
Subtilisins / genetics*

Substances

Protein Precursors
Subtilisins

Associated data

GENBANK/D10010
GENBANK/M55187
GENBANK/M55188
GENBANK/M91426
GENBANK/M91427
GENBANK/M91428
GENBANK/M91429
GENBANK/M91430
GENBANK/X60462
GENBANK/X62369