Recognition of human cytomegalovirus by human primary immunoglobulins identifies an innate foundation to an adaptive immune response

J Immunol. 2005 Apr 15;174(8):4768-78. doi: 10.4049/jimmunol.174.8.4768.

Abstract

Most primates, including humans, are chronically infected with cospecifically evolved, potentially pathogenic CMV. Abs that bind a 10-aa linear epitope (antigenic determinant 2 site 1) within the extracellular domain of human CMV glycoprotein B neutralize viral infectivity. In this study, we show that genes generated by recombinations involving two well-conserved human germline V elements (IGHV3-30 and IGKV3-11), and IGHJ4, encode primary Ig molecules that bind glycoprotein B at this key epitope. These particular V(H), J(H), and V(kappa) genes enable humans to generate through recombination and N nucleotide addition, a useful frequency of primary Igs that efficiently target this critical site on human CMV and thus confer an innate foundation for a specific adaptive response to this pathogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological
  • Amino Acid Sequence
  • Antibodies, Viral / chemistry
  • Antibodies, Viral / genetics
  • Antibodies, Viral / metabolism*
  • Antigens, Viral / genetics
  • Base Sequence
  • Codon / genetics
  • Cytomegalovirus / immunology*
  • DNA, Complementary / genetics
  • Humans
  • Immunity, Innate
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Light Chains / genetics
  • Immunoglobulin Variable Region / genetics
  • Immunoglobulins / chemistry
  • Immunoglobulins / genetics
  • Immunoglobulins / metabolism*
  • In Vitro Techniques
  • Molecular Sequence Data
  • Peptide Fragments / genetics
  • Peptide Fragments / immunology
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Codon
  • DNA, Complementary
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Immunoglobulin Variable Region
  • Immunoglobulins
  • Peptide Fragments
  • Recombinant Proteins
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus

Associated data

  • GENBANK/AJ606108
  • GENBANK/AJ606109
  • GENBANK/AJ606110
  • GENBANK/AJ606111
  • GENBANK/AJ606112
  • GENBANK/AJ606113
  • GENBANK/AJ606114
  • GENBANK/AJ606115
  • GENBANK/AJ606116
  • GENBANK/AJ606117
  • GENBANK/AJ606118
  • GENBANK/AJ606119
  • GENBANK/AJ606120
  • GENBANK/AJ606121
  • GENBANK/AJ606122
  • GENBANK/AJ606123