Structure of a gammadelta T cell receptor in complex with the nonclassical MHC T22

Science. 2005 Apr 8;308(5719):227-31. doi: 10.1126/science.1106885.

Abstract

Gammadelta T cell receptors (TCRs), alphabeta TCRs, and antibodies are the three lineages of somatically recombined antigen receptors. The structural basis for ligand recognition is well defined for alphabeta TCR and antibodies but is lacking for gammadelta TCRs. We present the 3.4 A structure of the murine gammadelta TCR G8 bound to its major histocompatibility complex (MHC) class Ib ligand, T22. G8 predominantly uses germline-encoded residues of its delta chain complementarity-determining region 3 (CDR3) loop to bind T22 in an orientation substantially different from that seen in alphabeta TCR/peptide-MHC. That junctionally encoded G8 residues play an ancillary role in binding suggests a fusion of innate and adaptive recognition strategies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Binding Sites
  • Cell Line
  • Cloning, Molecular
  • Crystallography, X-Ray
  • Dimerization
  • Histocompatibility Antigens Class I / chemistry*
  • Humans
  • Insecta
  • Mice
  • Protein Binding
  • Protein Conformation
  • Proteins / chemistry*
  • Proteins / immunology
  • Receptors, Antigen, T-Cell, gamma-delta / chemistry*
  • Receptors, Antigen, T-Cell, gamma-delta / immunology
  • Recombinant Proteins / chemistry
  • T-Lymphocytes / immunology

Substances

  • Histocompatibility Antigens Class I
  • Proteins
  • Receptors, Antigen, T-Cell, gamma-delta
  • Recombinant Proteins
  • T22 antigen

Associated data

  • PDB/1YPZ