The implication of follicular lymphoma patients receiving allogeneic stem cell transplantation from donors carrying t(14;18)-positive cells

Bone Marrow Transplant. 2005 Jun;35(11):1049-54. doi: 10.1038/sj.bmt.1704969.

Abstract

We performed real-time quantitative polymerase chain reaction (RQ-PCR) in peripheral blood (PB) and/or bone marrow (BM) samples collected pre- and post transplant from 23 recipient-donor pairs receiving allogeneic stem cell transplantation (allo-SCT) for follicular lymphoma (FL). Of 23 donors, 11 had a PB and/or BM sample positive for t(14;18) (BCL2/IGH fusion) at low levels (<one t(14;18) cell in 10K total cells). Recipients from donors with (n=11) and those without (n=12) detectable t(14:18) cells were similar in age, sex, and disease status pretransplant. No differences in the incidence of graft-versus-host-disease (GVHD), delayed engraftment, relapse rate, disease-free survival and overall survival were identified between the groups. Two recipients without detectable t(14;18) cells pre-transplant showed detectable t(14;18) cells at 2 and 11 years after receiving grafts from donors with t(14:18) cells. Neither patient developed FL 1.5 and 2 years after the emergence of t(14;18) cells. Although the sample size is relatively small, our findings suggest that individuals carrying t(14;18) cells may not be excluded as donors given the lack of an association of t(14;18) detected in donors with adverse clinical outcome. It may be necessary to screen for the donor's t(14;18) status before using t(14;18) for monitoring minimal residual disease by RQ-PCR to exclude the possibility of confounding donor's t(14;18) clone.

MeSH terms

  • Adult
  • Bone Marrow Cells / cytology
  • Chromosomes, Human, Pair 14 / ultrastructure*
  • Chromosomes, Human, Pair 18 / ultrastructure*
  • Disease-Free Survival
  • Female
  • Graft vs Host Disease
  • Humans
  • Lymphoma, Follicular / mortality
  • Lymphoma, Follicular / therapy*
  • Male
  • Middle Aged
  • Recurrence
  • Retrospective Studies
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stem Cell Transplantation / methods*
  • Time Factors
  • Tissue Donors
  • Translocation, Genetic*
  • Transplantation, Homologous / methods
  • Treatment Outcome