Progression of respiratory syncytial virus infection monitored by fluorescent quantum dot probes

Nano Lett. 2005 Apr;5(4):591-5. doi: 10.1021/nl048073u.

Abstract

We report the use of quantum dots (QDs) to identify the presence and monitor the progression of respiratory syncytial virus (RSV) infection over time by labeling the F and G proteins. In addition, co-localization of these viral proteins was shown using confocal microscopy. The implications of these results are that QDs may provide a method for early, rapid detection of viral infection and open the door for future studies of the intricate spatial features cell trafficking of viral proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Fluorescent Dyes*
  • Humans
  • Quantum Dots*
  • Respiratory Syncytial Virus Infections / diagnosis*
  • Respiratory Syncytial Virus, Human / physiology
  • Viral Envelope Proteins / analysis*
  • Viral Envelope Proteins / chemistry
  • Viral Fusion Proteins / analysis*
  • Viral Fusion Proteins / chemistry
  • Virus Replication / physiology

Substances

  • Fluorescent Dyes
  • Viral Envelope Proteins
  • Viral Fusion Proteins
  • attachment protein G