CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines

Giuseppe Sconocchia; Maurizio Provenzano; Katayoun Rezvani; Jongming Li; Jos Melenhorst; Nancy Hensel; A John Barrett

doi:10.1186/1479-5876-3-15

CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines

J Transl Med. 2005 Apr 14;3(1):15. doi: 10.1186/1479-5876-3-15.

Authors

Giuseppe Sconocchia¹, Maurizio Provenzano, Katayoun Rezvani, Jongming Li, Jos Melenhorst, Nancy Hensel, A John Barrett

Affiliation

¹ Hematology Branch, Stem Cell Allotransplantation Section, National Hearth Lung and Blood Institute, Bethesda, MD, USA. [email protected].

Abstract

In vitro stimulation of CD34+ cells with IL-2 induces NK cell differentiation. In order to define the stages of NK cell development, which influence their generation from CD34 cells, we cultured G-CSF mobilized peripheral blood CD34+ cells in the presence of stem cell factor and IL-2. After three weeks culture we found a diversity of CD56+ subsets which possessed granzyme A, but lacked the cytotoxic apparatus required for classical NK-like cytotoxicity. However, these CD56+ cells had the unusual property of inhibiting proliferation of K562 and P815 cell lines in a cell-contact dependent fashion.