Antimicrobial susceptibility of 79 non-typable Haemophilus influenzae isolations obtained from healthy children that attended two day-care centers in Marianao municipality. It was found resistance to trimetoprim/sulfamethoxazole (41,77 %), tetracycline (18,99), ampicilline (17,72 %), amoxicillin/ clavulanic acid (7,59 %) and chloramphenicol (6,33 %). 25,81 % of isolates showed multiresistance. 100 % of studied cases was sensitive to ceftriaxone, cefuroxime and norfloxacin. 28,57 % of ampicilline-resistent isolates produced beta-lactamase enzyme. Chloramphenicol resistance was mediated by the production of chloramphenicol acetyltransferase enzyme.