Abstract
Deletion of PDR5 gene (Deltapdr5) in Saccharomyces cerevisiae led to increased resistance to calcium. The cellular Ca2+ level in the presence of high calcium as estimated by reporter assay in Deltapdr5 cells was significantly lower than that in wild-type cells. Membrane Pdr5p levels diminished rapidly during incubation with high calcium in a manner dependent on calcineurin and Pep4p, suggesting a feedback regulatory mechanism for Pdr5p abundance.
MeSH terms
-
ATP-Binding Cassette Transporters / genetics
-
ATP-Binding Cassette Transporters / metabolism*
-
Calcium / metabolism*
-
Calcium / pharmacology
-
Gene Expression Regulation, Fungal / drug effects
-
Homeostasis*
-
Mutation / genetics
-
Saccharomyces cerevisiae / drug effects
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / metabolism*
-
Saccharomyces cerevisiae Proteins / genetics
-
Saccharomyces cerevisiae Proteins / metabolism*
Substances
-
ATP-Binding Cassette Transporters
-
PDR5 protein, S cerevisiae
-
Saccharomyces cerevisiae Proteins
-
Calcium