Abstract
Using a two-step procedure involving insertional gene targeting and recombinase-mediated cassette exchange in ES cells, we have produced two lines of transgenic mice expressing a dominant-negative latency-associated myostatin propeptide under control of the myosin light chain 1F promoter and 1/3 enhancer from the TSPY locus on the Y chromosome. Males of the corresponding lines are characterized by a 5-20% increase in skeletal muscle mass. This experiment demonstrates the feasibility of a more efficient cattle production system combining superior beef production abilities for bulls and dairy abilities for cows.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Body Weights and Measures
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Breeding / methods
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DNA-Binding Proteins / genetics
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Gene Targeting / methods*
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Genetic Engineering / methods
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Genetic Vectors / genetics
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Male
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Mice
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Mice, Transgenic
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Muscle Development / genetics*
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Muscle, Skeletal / growth & development*
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Myosin Light Chains / genetics*
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Myostatin
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Nuclear Proteins / genetics
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Promoter Regions, Genetic / genetics
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Sex-Determining Region Y Protein
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Totipotent Stem Cells / metabolism
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Transcription Factors / genetics
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Transforming Growth Factor beta / genetics
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Transforming Growth Factor beta / metabolism*
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Y Chromosome / genetics*
Substances
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DNA-Binding Proteins
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Mstn protein, mouse
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Myosin Light Chains
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Myostatin
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Nuclear Proteins
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Sex-Determining Region Y Protein
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Transcription Factors
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Transforming Growth Factor beta
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myosin light chain I