Increased expression of osteopontin is associated with long-term bee venom immunotherapy

J Allergy Clin Immunol. 2005 May;115(5):1063-7. doi: 10.1016/j.jaci.2005.01.055.

Abstract

Background: Venom allergen immunotherapy (VIT) is proven to be highly effective for insect allergy, but the mechanisms and the biomarkers associated with clinical efficacy remain elusive.

Objective: The aim of this study was to identify candidate biomarkers associated with successful VIT.

Methods: Gene chip array and clustering analyses of PBMCs from subjects with or without VIT were performed.

Results: From gene chip array and clustering analyses, an increased expression of osteopontin was found in patients who completed 5 to 6 years of VIT and discontinued therapy for 3 to 6 years (completed treatment group) compared with the untreated group. A significantly higher level of serum osteopontin was found in the completed treatment group compared with the untreated group (n = 16 in each group; P < .001).

Conclusion: The upregulation of osteopontin after VIT suggests a role of osteopontin as a candidate biomarker for VIT.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Animals
  • Bees
  • Biomarkers / blood
  • Cells, Cultured
  • Cytokines / blood
  • Cytokines / genetics
  • Cytokines / metabolism*
  • Female
  • Humans
  • Hypersensitivity / blood
  • Hypersensitivity / therapy*
  • Immunotherapy*
  • Leukocytes, Mononuclear / immunology
  • Male
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis
  • Osteopontin
  • RNA / analysis
  • RNA, Messenger / analysis
  • Sialoglycoproteins / blood
  • Sialoglycoproteins / genetics
  • Sialoglycoproteins / metabolism*
  • Time Factors
  • Venoms / therapeutic use*

Substances

  • Biomarkers
  • Cytokines
  • RNA, Messenger
  • SPP1 protein, human
  • Sialoglycoproteins
  • Venoms
  • Osteopontin
  • RNA