Structure of an acidic polysaccharide from the marine bacterium Pseudoalteromonas aliena type strain KMM 3562T containing two residues of L-serine in the repeating unit

Eva-Lisa Nordmark; Andrei V Perepelov; Alexander S Shashkov; Evgeny L Nazarenko; Raisa P Gorshkova; Elena P Ivanova; Göran Widmalm

doi:10.1016/j.carres.2005.03.013

Structure of an acidic polysaccharide from the marine bacterium Pseudoalteromonas aliena type strain KMM 3562T containing two residues of L-serine in the repeating unit

Carbohydr Res. 2005 Jun 13;340(8):1483-7. doi: 10.1016/j.carres.2005.03.013.

Authors

Eva-Lisa Nordmark¹, Andrei V Perepelov, Alexander S Shashkov, Evgeny L Nazarenko, Raisa P Gorshkova, Elena P Ivanova, Göran Widmalm

Affiliation

¹ Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.

PMID: 15882858
DOI: 10.1016/j.carres.2005.03.013

Abstract

The structure of an acidic polysaccharide from Pseudoalteromonas aliena type strain KMM 3562(T) has been elucidated. The polysaccharide was studied by component analysis, (1)H and (13)C NMR spectroscopy, including 2D NMR experiments. A (1)H, (13)C band-selective constant-time heteronuclear multiple-bond connectivity experiment was used to determine amide linkages, between serine and uronic acid (UA) residues, via (3)J(H,C) correlations between Ser-alphaH and UA-C-6. It was found that the polysaccharide consists of pentasaccharide repeating units with the following structure: [carbohydrate structure]; see text.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Polysaccharides / chemistry*
Polysaccharides / isolation & purification
Pseudoalteromonas / chemistry*
Pseudoalteromonas / classification*
Serine / analysis*

Substances

Polysaccharides
Serine