Isolation and functional characterization of siderophore-producing lead- and cadmium-resistant Pseudomonas putida KNP9

Curr Microbiol. 2005 May;50(5):233-7. doi: 10.1007/s00284-004-4459-4. Epub 2005 Apr 18.

Abstract

Heavy metals, being phytotoxic, cause growth inhibition and even plant death. Siderophore-producing bacterial strain KNP9 is growth promoting and has been isolated from Panki Power Plant, Kanpur, India. It simulated significant (p > 5%) root and shoot growth of mung bean to the extent of 16.48% and 28.80%, respectively in the presence of CdCl(2) (110 microM: ). However, the increase in root and shoot growth was 20% and 19.5%, respectively, in the presence of (CH(3)COO)(2)Pb (660 microM: ). Moreover, concentration of accumulated lead and cadmium in root and shoot was also reduced in the presence of this isolate ranging from 37.5 to 93.19%. A moderate reduction in chlorophyll content (39.14%) in the presence of 110 microM: CdCl(2) was rescued by bioinoculant KNP9. However, the 19.58% decrease in chlorophyll content in the case of lead acetate remained unchanged even in the presence of KNP9. Nevertheless, 16S ribosomal DNA (rDNA) sequencing identified KNP9 as a strain of Pseudomonas putida.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cadmium / analysis
  • Cadmium Chloride
  • DNA, Bacterial / analysis
  • Drug Resistance, Bacterial / genetics
  • Lead / analysis
  • Organometallic Compounds
  • Phaseolus / chemistry
  • Phaseolus / growth & development*
  • Phaseolus / microbiology
  • Plant Growth Regulators / metabolism*
  • Plant Roots / chemistry
  • Plant Roots / growth & development
  • Plant Roots / microbiology
  • Plant Shoots / chemistry
  • Plant Shoots / growth & development
  • Plant Shoots / microbiology
  • Power Plants
  • Pseudomonas / genetics
  • Pseudomonas / isolation & purification
  • Pseudomonas / metabolism*
  • Siderophores / metabolism*
  • Soil Microbiology*
  • Soil Pollutants*

Substances

  • DNA, Bacterial
  • Organometallic Compounds
  • Plant Growth Regulators
  • Siderophores
  • Soil Pollutants
  • Cadmium
  • Lead
  • Cadmium Chloride
  • lead acetate