Abstract
Immature dendritic cells (DCs) derived from freshly isolated human monocytes were used to evaluate the effect of the inhibiting receptor CD85j (leukocyte Ig-like receptor-1/ILT2) on activation induced by cross-linking of the human osteoclast-associated receptor (hOSCAR). CD85j and hOSCAR were expressed consistently at the same density on monocytes and on monocyte-derived DCs (both immature and mature). Cross-linking of hOSCAR, which activates via the FcR-associated gamma-chain, induced Ca(2+) flux in DCs. Concomitant cross-linking of anti-CD85j mAb abolished this early activation event. Likewise, CD85j stimulation strongly reduced IL-8 and IL-12 production by hOSCAR-activated DCs. Inhibition of DCs via CD85j also impaired their ability to enhance Ag-specific T cell proliferation induced by hOSCAR. Finally, because hOSCAR prevents apoptosis of DCs in the absence of growth/survival factors, CD85j cross-linking was able to counteract completely this antiapoptotic effect and to reduce Bcl-2 expression enhanced by hOSCAR stimulation. Thus, CD85j is an inhibiting receptor that is functional in human DCs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD / biosynthesis
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Antigens, CD / physiology*
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Apoptosis / immunology
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Calcium / antagonists & inhibitors
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Calcium / metabolism
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Calcium Signaling / immunology
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Cell Differentiation / immunology
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Cell Separation
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Cells, Cultured
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Cytokines / antagonists & inhibitors
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Cytokines / biosynthesis
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Dendritic Cells / cytology
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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Down-Regulation / immunology
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Epitopes, T-Lymphocyte / immunology
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Growth Inhibitors / physiology*
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Humans
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Leukocyte Immunoglobulin-like Receptor B1
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Lymphocyte Activation / immunology
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Monocytes / cytology
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Monocytes / immunology
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Monocytes / metabolism
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Osteoclasts / immunology
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Osteoclasts / metabolism
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Receptors, Cell Surface / antagonists & inhibitors*
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Receptors, Cell Surface / biosynthesis
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Receptors, Cell Surface / physiology*
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Receptors, Immunologic / biosynthesis
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Receptors, Immunologic / physiology*
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T-Lymphocyte Subsets / immunology
Substances
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Antigens, CD
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Cytokines
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Epitopes, T-Lymphocyte
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Growth Inhibitors
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LILRB1 protein, human
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Leukocyte Immunoglobulin-like Receptor B1
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OSCAR protein, human
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Receptors, Cell Surface
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Receptors, Immunologic
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Calcium