Protein microarray for profiling antibody responses to Yersinia pestis live vaccine

Bei Li; Lingxiao Jiang; Qifeng Song; Junxin Yang; Zeliang Chen; Zhaobiao Guo; Dongsheng Zhou; Zongmin Du; Yajun Song; Jin Wang; Hongxia Wang; Shouyi Yu; Jian Wang; Ruifu Yang

doi:10.1128/IAI.73.6.3734-3739.2005

Protein microarray for profiling antibody responses to Yersinia pestis live vaccine

Infect Immun. 2005 Jun;73(6):3734-9. doi: 10.1128/IAI.73.6.3734-3739.2005.

Authors

Bei Li¹, Lingxiao Jiang, Qifeng Song, Junxin Yang, Zeliang Chen, Zhaobiao Guo, Dongsheng Zhou, Zongmin Du, Yajun Song, Jin Wang, Hongxia Wang, Shouyi Yu, Jian Wang, Ruifu Yang

Affiliation

¹ Laboratory of Analytical Microbiology, National Center for Biomedical Analysis, Army Center for Microbial Detection and Research, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.

Abstract

A protein microarray representing 149 Yersinia pestis proteins was developed to profile antibody responses in EV76-immunized rabbits. Antibodies to 50 proteins were detected. There are 11 proteins besides F1 and V antigens to which the predominant antibody response occurred, and these proteins show promise for further evaluation as candidates for subunit vaccines and/or diagnostic antigens.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Bacterial / blood*
Antigens, Bacterial / immunology
Immunization
Plague Vaccine / immunology*
Protein Array Analysis*
Rabbits
Yersinia pestis / immunology*

Substances

Antibodies, Bacterial
Antigens, Bacterial
Plague Vaccine