Abstract
The basis of the rough-to-smooth conversion of Actinobacillus actinomycetemcomitans was examined. Smooth variants often contained mutations at the flp promoter region. Replacing the mutated flp promoter with the wild-type promoter restored the rough phenotype. The expression level of the flp promoter was approximately 100-fold lower in smooth than in rough strains. Mutations of the flp promoter are a cause of the rough-to-smooth conversion.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aggregatibacter actinomycetemcomitans / cytology*
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Aggregatibacter actinomycetemcomitans / genetics*
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Aggregatibacter actinomycetemcomitans / ultrastructure
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Bacterial Proteins / genetics
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Base Sequence
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Microscopy, Electron, Transmission
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Molecular Sequence Data
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Promoter Regions, Genetic
Substances
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Bacterial Proteins
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Flp protein, bacteria