Abstract
An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alcohol Oxidoreductases / chemistry
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Alcohol Oxidoreductases / metabolism*
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Aldehyde Reductase / metabolism
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Aldo-Keto Reductases
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Ligands
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Microscopy, Fluorescence
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Models, Molecular
Substances
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Ligands
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Alcohol Oxidoreductases
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Aldo-Keto Reductases
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Aldehyde Reductase