Expression of T cell immunoglobulin- and mucin-domain-containing molecules-1 and -3 (TIM-1 and -3) in the rat nervous and immune systems

Alexander W Gielen; Anna Lobell; Olle Lidman; Mohsen Khademi; Tomas Olsson; Fredrik Piehl

doi:10.1016/j.jneuroim.2005.04.004

Expression of T cell immunoglobulin- and mucin-domain-containing molecules-1 and -3 (TIM-1 and -3) in the rat nervous and immune systems

J Neuroimmunol. 2005 Jul;164(1-2):93-104. doi: 10.1016/j.jneuroim.2005.04.004.

Authors

Alexander W Gielen¹, Anna Lobell, Olle Lidman, Mohsen Khademi, Tomas Olsson, Fredrik Piehl

Affiliation

¹ Department of Clinical Neuroscience, Neuroimmunology Unit, CMM, L8:04, Karolinska University Hospital, Karolinska Institutet, SE 171 76, Stockholm, Sweden. [email protected]

PMID: 15913792
DOI: 10.1016/j.jneuroim.2005.04.004

Abstract

Expression of T cell immunoglobulin- and mucin-domain-containing molecules (TIMs) can be used as T helper (Th) differentiation markers in the human and mouse. We examined the expression of TIM-1 and -3 mRNAs in rat MBP(63-88)-induced experimental autoimmune encephalomyelitis (EAE). TIM-3 expression was upregulated in the spinal cord during EAE and following antigen restimulation of the encephalitogenic TCRBV8S2+ population. Interestingly, TIM-3 expression was also detected by in situ hybridization in resident cells of the nervous system. TIM-1 was expressed in B cells but not in resident CNS cells and TIM-1 mRNA levels in spinal cord were unchanged throughout the course of EAE. These results support the notion that TIM-3 can also be used as a Th1 differentiation marker in the rat. However, expression of TIM-1 and -3 is not restricted solely to T cells and the presence of TIM-3 in resident CNS cells may indicate a role for this molecule in the interaction between the nervous and immune systems.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Encephalomyelitis, Autoimmune, Experimental / chemically induced
Encephalomyelitis, Autoimmune, Experimental / metabolism*
Female
Flow Cytometry / methods
Gene Expression Regulation / physiology*
Hepatitis A Virus Cellular Receptor 1
Hepatitis A Virus Cellular Receptor 2
Immune System / metabolism*
In Situ Hybridization / methods
Interferon-gamma / genetics
Interferon-gamma / metabolism
Interleukin-10 / genetics
Interleukin-10 / metabolism
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Myelin Basic Protein
Nervous System / metabolism*
Nervous System / pathology
Peptide Fragments
RNA, Messenger / biosynthesis
Rats
Rats, Inbred Lew
Receptors, Virus / genetics
Receptors, Virus / metabolism*
Reverse Transcriptase Polymerase Chain Reaction / methods

Substances

Havcr1 protein, mouse
Havcr2 protein, mouse
Hepatitis A Virus Cellular Receptor 1
Hepatitis A Virus Cellular Receptor 2
Membrane Proteins
Myelin Basic Protein
Peptide Fragments
RNA, Messenger
Receptors, Virus
Interleukin-10
Interferon-gamma