Abstract
The lambda 5 gene is a homolog of immunoglobulin J lambda-C lambda genes, expressed specifically in immature B-lineage cells. Lambda 5-encoded molecules form membrane complexes with mu or D mu proteins in association with an additional protein specifically expressed in immature B cells that is encoded by the Vpre-B gene. We have generated mice in which the lambda 5 gene is inactivated by targeted gene disruption in embryonic stem cells. In these mice, B cell development in the bone marrow is blocked at the pre-B cell stage. However, the blockade is leaky, allowing B cells to populate the peripheral immune system at a low rate. These cells are allelically excluded and able to respond to antigen.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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B-Lymphocytes / cytology*
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B-Lymphocytes / immunology
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Base Sequence
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Cell Differentiation / genetics
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Female
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Genes, Immunoglobulin / genetics*
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Hematopoietic Stem Cells / cytology*
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Hematopoietic Stem Cells / immunology
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Humans
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Immunoglobulin Constant Regions / genetics*
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Immunoglobulin Joining Region / genetics*
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Immunoglobulin Variable Region / genetics
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Immunoglobulin lambda-Chains / genetics*
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Male
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Mutagenesis, Insertional
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Mutation / genetics
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Neomycin / pharmacology
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Polymerase Chain Reaction
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Recombination, Genetic / genetics
Substances
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Immunoglobulin Constant Regions
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Immunoglobulin Joining Region
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Immunoglobulin Variable Region
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Immunoglobulin lambda-Chains
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Neomycin