Comment to Shinohara et al. (1991) FEBS Letters 293, 173-174. The uncoupling protein is not expressed in rat liver

D Ricquier; S Raimbault; O Champigny; B Miroux; F Bouillaud

doi:10.1016/0014-5793(92)80496-4

Comment to Shinohara et al. (1991) FEBS Letters 293, 173-174. The uncoupling protein is not expressed in rat liver

FEBS Lett. 1992 May 25;303(1):103-6; discussion 107. doi: 10.1016/0014-5793(92)80496-4.

Authors

D Ricquier¹, S Raimbault, O Champigny, B Miroux, F Bouillaud

Affiliation

¹ Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement, Centre National de la Recherche Scientifique, Meudon, France.

PMID: 1592106
DOI: 10.1016/0014-5793(92)80496-4

Abstract

Using Northern blot analysis, immunoblotting with purified antibodies and Polymerase Chain Reaction analysis, we were unable to detect the Uncoupling Protein-UCP or its mRNA in liver of control, cold-exposed or newborn rats. The unique expression of this protein in brown adipocytes was confirmed. These data refute the surprising recent report on UCP expression in rat liver (Shinohara (1991) FEBS Lett. 293, 173-174). Moreover we report that the hybridization signal obtained by these authors is probably non-specific and due to the 3' non-coding domain of the UCP cDNA probe.

Publication types

Research Support, Non-U.S. Gov't
Comment

MeSH terms

Animals
Blotting, Northern
Blotting, Western
Carrier Proteins / genetics*
Carrier Proteins / metabolism
DNA / genetics
DNA Probes
Ion Channels
Male
Membrane Proteins / genetics*
Membrane Proteins / metabolism
Mitochondria, Liver / metabolism*
Mitochondrial Proteins
Polymerase Chain Reaction
Rats
Rats, Inbred Strains
Tissue Distribution
Transcription, Genetic
Uncoupling Protein 1

Substances

Carrier Proteins
DNA Probes
Ion Channels
Membrane Proteins
Mitochondrial Proteins
Uncoupling Protein 1
DNA