H7, a protein kinase C inhibitor, increases the glutathione content of neuroblastoma cells

FEBS Lett. 1992 May 25;303(1):19-21. doi: 10.1016/0014-5793(92)80468-v.

Abstract

It is shown that the intracellular glutathione (GSH) concentration of neuroblastoma-2a cells in culture increases with a maximum at 24 h after starting treatment with 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), an inhibitor of protein kinase C (PKC). Other inhibitors of this and other protein kinases, e.g. sphingosine, staurosporine, and HA 1004, at the concentrations tested, had a less marked or negligible effect on intracellular GSH concentration. 12-O-Tetradecanoylphorbol-13-acetate (TPA) was also tested and showed no significant effect 24 h after addition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Alkaloids / pharmacology
  • Animals
  • Enzyme Activation
  • Glutathione / biosynthesis*
  • Isoquinolines / pharmacology*
  • Mice
  • Neuroblastoma / metabolism*
  • Piperazines / pharmacology*
  • Protein Kinase C / antagonists & inhibitors*
  • Protein Kinase C / metabolism
  • Sphingosine / pharmacology
  • Staurosporine
  • Sulfonamides*
  • Tumor Cells, Cultured / drug effects*

Substances

  • Alkaloids
  • Isoquinolines
  • Piperazines
  • Sulfonamides
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • N-(2-guanidinoethyl)-5-isoquinolinesulfonamide
  • Protein Kinase C
  • Glutathione
  • Staurosporine
  • Sphingosine