Abstract
The dipole potential (Psi(d)) constitutes a large and functionally important part of the electrostatic potential of cell plasma membranes. However, its direct measurement is not possible. Herein, new 3-hydroxyflavone fluorescent probes were developed that respond strongly to Psi(d) changes by a variation of the intensity ratio of their two well-separated fluorescence bands. Using fluorescence spectroscopy with cell suspensions and confocal microscopy with adherent cells, we showed, for the first time, two-color fluorescence ratiometric measurement and visualization of Psi(d) in cell plasma membranes. Using this new tool, a heterogeneous distribution of this potential within the membrane was evidenced.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Adhesion
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Cell Line
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Cell Line, Tumor
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Cell Membrane / metabolism*
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Dose-Response Relationship, Drug
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Fibroblasts / metabolism
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Flavonoids / chemistry
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Flavonoids / metabolism
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Fluorescent Dyes / pharmacology*
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Humans
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Hydrogen-Ion Concentration
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Ketocholesterols / chemistry
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Lipid Bilayers / chemistry
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Lipids / chemistry
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Membrane Potentials
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Mice
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Microscopy, Confocal / methods*
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Microscopy, Fluorescence / methods*
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Models, Chemical
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Phosphatidylcholines / chemistry
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Phosphatidylglycerols / chemistry
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Protons
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Pyridinium Compounds / pharmacology
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Static Electricity
Substances
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1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine
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Flavonoids
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Fluorescent Dyes
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Ketocholesterols
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Lipid Bilayers
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Lipids
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Phosphatidylcholines
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Phosphatidylglycerols
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Protons
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Pyridinium Compounds
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6-ketocholestanol
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1,2-dioleoyl-sn-glycero-3-phosphoglycerol
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1,2-oleoylphosphatidylcholine
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3-hydroxyflavone