Investigation of the between-gel and within-gel variation in fragment size determinations found when using single locus DNA probes

D Syndercombe Court; T Fedor; M Gouldstone; P J Lincoln; C P Phillips; V Tate; J A Thomson; P W Watts

doi:10.1016/0379-0738(92)90194-2

Investigation of the between-gel and within-gel variation in fragment size determinations found when using single locus DNA probes

Forensic Sci Int. 1992 Mar;53(2):173-91. doi: 10.1016/0379-0738(92)90194-2.

Authors

D Syndercombe Court¹, T Fedor, M Gouldstone, P J Lincoln, C P Phillips, V Tate, J A Thomson, P W Watts

Affiliation

¹ Department of Haematology, London Hospital Medical College, UK.

PMID: 1592327
DOI: 10.1016/0379-0738(92)90194-2

Abstract

Measurement variation in the sizing of DNA fragments has been assessed, examining within-gel and between-gel variability. Also, measurement variation detected between two different laboratories, using both manual and automated measurement techniques, has been investigated and discussed.

Publication types

Comparative Study

MeSH terms

Analysis of Variance
Autoradiography
DNA / chemistry*
DNA Probes*
Electrophoresis, Agar Gel
Female
Humans
Image Processing, Computer-Assisted
Mothers
Nucleic Acid Hybridization
Repetitive Sequences, Nucleic Acid
Reproducibility of Results

Substances

DNA Probes
DNA