Abstract
We present a convenient method for monitoring pancreatic beta cell development in real-time, through in vitro culture of embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Culture Techniques / methods
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Green Fluorescent Proteins / metabolism*
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Islets of Langerhans / cytology*
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Islets of Langerhans / embryology*
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Mice
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Mice, Transgenic
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Microscopy, Fluorescence
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Transgenes / genetics
Substances
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Green Fluorescent Proteins