Objective: To establish anti-heterogeneous nuclear ribonucleoprotein B1 monoclonal antibody cell line and to identify immunological activity.
Methods: BALB/c mice were immunized with specific hnRNPB1 polypeptide by multiple-place hypodermic injection. The splenic cells of the mice were fused with SP2/0 mouse myeloma cells. The hybridoma culture supernants were screened by indirect ELISA with artificial synthetic peptide.
Results: Two hybridoma cell lines against hnRNPB1 were successfully obtained; the titers of antibody in ascites were 1: 1.8 x 10(5). The McAb showed the character of no cross reactions. Immunohistochemical analysis demonstrated that the antibody could bind with hnRNPB1 specifically.
Conclusion: The McAb prepared so by this laboratory is of good specificity and sensitivity.