Identification of Helicobacter pylori surface proteins by selective proteinase K digestion and antibody phage display

Nicolas Sabarth; Robert Hurvitz; Monika Schmidt; Ursula Zimny-Arndt; Peter R Jungblut; Thomas F Meyer; Dirk Bumann

doi:10.1016/j.mimet.2005.04.030

Identification of Helicobacter pylori surface proteins by selective proteinase K digestion and antibody phage display

J Microbiol Methods. 2005 Sep;62(3):345-9. doi: 10.1016/j.mimet.2005.04.030.

Authors

Nicolas Sabarth¹, Robert Hurvitz, Monika Schmidt, Ursula Zimny-Arndt, Peter R Jungblut, Thomas F Meyer, Dirk Bumann

Affiliation

¹ Max Planck Institute for Infection Biology, Department of Molecular Biology, Schumannstrasse 21/22, D-10117 Berlin, Germany. [email protected]

PMID: 15939494
DOI: 10.1016/j.mimet.2005.04.030

Abstract

Five surface proteins of Helicobacter pylori were identified by proteinase K treatment of live H. pylori followed by proteome analysis. One of the identified proteins, HopQ, is also recognized by an antibody selected by phage display screening of intact H. pylori.

MeSH terms

Animals
Antibodies, Bacterial / genetics
Antibodies, Monoclonal / genetics
Bacterial Outer Membrane Proteins / genetics
Bacterial Outer Membrane Proteins / immunology
Bacterial Outer Membrane Proteins / isolation & purification*
Bacteriological Techniques
Electrophoresis, Gel, Two-Dimensional
Endopeptidase K
Helicobacter pylori / chemistry*
Helicobacter pylori / genetics
Helicobacter pylori / immunology
Mice
Molecular Sequence Data
Peptide Library
Proteomics

Substances

Antibodies, Bacterial
Antibodies, Monoclonal
Bacterial Outer Membrane Proteins
Peptide Library
Endopeptidase K

Associated data

GENBANK/AF536561