Abstract
Delivery of oligonucleotides has been a major impediment in the development of nucleic acid based drugs. In this report, we show that neomycin, an aminoglycoside antibiotic, when combined with a cationic lipid preparation such as DOTAP, enhances transfection efficiency of both reporter plasmids and oligonucleotides and results in a significant increase in transgene expression. The results described here open a new lead in ongoing efforts for oligonucleotide delivery.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbohydrate Sequence
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Cations / chemistry
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Cell Line, Tumor
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DNA / chemistry*
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Drug Delivery Systems
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Fatty Acids, Monounsaturated / chemistry
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Humans
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Lipids / chemistry*
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Male
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Molecular Sequence Data
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Molecular Structure
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Neomycin / chemistry*
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Prostatic Neoplasms / metabolism*
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Quaternary Ammonium Compounds / chemistry
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Transfection / methods*
Substances
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Cations
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Fatty Acids, Monounsaturated
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Lipids
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Quaternary Ammonium Compounds
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DNA
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Neomycin
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1,2-dioleoyloxy-3-(trimethylammonium)propane