Multicenter evaluation of a Candida albicans peptide nucleic acid fluorescent in situ hybridization probe for characterization of yeast isolates from blood cultures

D A Wilson; M J Joyce; L S Hall; L B Reller; G D Roberts; G S Hall; B D Alexander; G W Procop

doi:10.1128/JCM.43.6.2909-2912.2005

Multicenter evaluation of a Candida albicans peptide nucleic acid fluorescent in situ hybridization probe for characterization of yeast isolates from blood cultures

J Clin Microbiol. 2005 Jun;43(6):2909-12. doi: 10.1128/JCM.43.6.2909-2912.2005.

Authors

D A Wilson¹, M J Joyce, L S Hall, L B Reller, G D Roberts, G S Hall, B D Alexander, G W Procop

Affiliation

¹ Section of Clinical Microbiology, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.

Abstract

We evaluated aliquots from 244 clinical blood culture bottles that demonstrated yeasts on Gram stain using a Candida albicans peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) probe. The sensitivity, specificity, positive predictive value, and negative predictive value of the C. albicans PNA FISH test in this study were 99%, 100%, 100%, and 99.3%, respectively.

Publication types

Evaluation Study
Multicenter Study

MeSH terms

Blood / microbiology
Candida albicans / classification*
Candida albicans / genetics
Candida albicans / isolation & purification
Candidiasis / microbiology
Culture Media
Fungemia / microbiology*
Humans
In Situ Hybridization, Fluorescence* / methods
Nucleic Acid Probes / genetics*
Peptide Nucleic Acids / genetics*
Predictive Value of Tests
Sensitivity and Specificity

Substances

Culture Media
Nucleic Acid Probes
Peptide Nucleic Acids