Use of genotypic identification by sodA sequencing in a prospective study to examine the distribution of coagulase-negative Staphylococcus species among strains recovered during septic orthopedic surgery and evaluate their significance

V Sivadon; M Rottman; S Chaverot; J-C Quincampoix; V Avettand; P de Mazancourt; L Bernard; P Trieu-Cuot; J-M Féron; A Lortat-Jacob; P Piriou; T Judet; J-L Gaillard

doi:10.1128/JCM.43.6.2952-2954.2005

Use of genotypic identification by sodA sequencing in a prospective study to examine the distribution of coagulase-negative Staphylococcus species among strains recovered during septic orthopedic surgery and evaluate their significance

J Clin Microbiol. 2005 Jun;43(6):2952-4. doi: 10.1128/JCM.43.6.2952-2954.2005.

Authors

V Sivadon¹, M Rottman, S Chaverot, J-C Quincampoix, V Avettand, P de Mazancourt, L Bernard, P Trieu-Cuot, J-M Féron, A Lortat-Jacob, P Piriou, T Judet, J-L Gaillard

Affiliation

¹ Laboratoire de Microbiologie, Hôpital Raymond Poincaré (AP-HP), 104 Bd Raymond Poincaré, 92380 Garches, France. [email protected]

Abstract

A total of 212 coagulase-negative Staphylococcus strains recovered prospectively during 119 surgeries for proven or suspected bone and joint infection (BJI) were identified by sodA sequencing. These strains were identified as 151 Staphylococcus epidermidis isolates, 15 S. warneri isolates, 14 S. capitis isolates, 9 S. hominis isolates, 6 S. lugdunensis isolates, 5 S. haemolyticus isolates, 4 S. caprae isolates, 4 S. pasteuri isolates, 3 S. simulans isolates, and 1 S. cohnii isolate. Only S. epidermidis, S. lugdunensis, S. capitis, and S. caprae were found to be infecting organisms and were involved, respectively, in 35 (81.4%), 3 (7.0%), 3 (7.0%), and 2 (4.6%) cases of BJI.

MeSH terms

Adult
Aged
Aged, 80 and over
Arthroplasty / adverse effects
Bacterial Proteins / genetics*
Bacterial Typing Techniques
Bone Diseases, Infectious / microbiology
Bone Diseases, Infectious / surgery*
Coagulase / metabolism*
Female
Genotype
Humans
Joint Diseases / microbiology
Joint Diseases / surgery*
Male
Middle Aged
Orthopedics / methods
Prospective Studies
Reoperation
Staphylococcal Infections / microbiology*
Staphylococcus / classification*
Staphylococcus / enzymology
Staphylococcus / genetics
Staphylococcus / isolation & purification*
Superoxide Dismutase / genetics*

Substances

Bacterial Proteins
Coagulase
SodA protein, Bacteria
Superoxide Dismutase