Use of the modified robbins device and fluorescent staining to screen plant extracts for the inhibition of S. mutans biofilm formation

K Honraet; H J Nelis

doi:10.1016/j.mimet.2005.05.005

Use of the modified robbins device and fluorescent staining to screen plant extracts for the inhibition of S. mutans biofilm formation

J Microbiol Methods. 2006 Feb;64(2):217-24. doi: 10.1016/j.mimet.2005.05.005. Epub 2005 Jun 23.

Authors

K Honraet¹, H J Nelis

Affiliation

¹ Laboratory for Pharmaceutical Microbiology, University of Ghent, Harelbekestraat 72, B-9000, Ghent, Belgium.

PMID: 15979176
DOI: 10.1016/j.mimet.2005.05.005

Abstract

Streptococcus mutans plays an important role in the formation of dental plaque. To study biofilm growth on hydroxyapatite (HA) in vitro, a flow system based on a Modified Robbins Device (MRD) and a method for the quantification of the biomass using fluorescent staining with SYTO(R) 9 were developed. The combined approach was used to assess the inhibitory effect of plant extracts on biofilm formation in concentrations below their minimal inhibitory concentrations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biofilms / growth & development*
Colony Count, Microbial / methods*
Durapatite
Fluorescent Dyes
Organic Chemicals
Streptococcus mutans / isolation & purification
Streptococcus mutans / physiology*

Substances

Fluorescent Dyes
Organic Chemicals
SYTO 9
Durapatite